Dihydroartemisinin is a newer approach to the treatment of Malaria. It is available in the combination with Piperaquine Phosphate in various dosage forms. Here, DHA has no strong UV absorbing groups and it is optically alive. It converts from α-DHA to β-DHA in organic solvents. Simultaneous estimation of these both drug is not possible, therefore these drugs are estimated separately in their formulation. The DHA was determined by RP-HPLC method by using Kromasil C18 (250 mm x 4.6 mm i.d., 5 µm particle size) column. A mobile phase composed of acetonitrile, Methanol, Water in proportion of 60:20:20 v/v, at flow rate of 0.8 ml/min was used for the separation. Detection was carried out at 215 nm. PQP was determined by using Acetonitrile, 0.1 mM phosphate buffer, 1 ml triethylamine (pH-2.5 is adjusted with o-phosphoric acid). Methods were validated statistically and recovery studies were carried out. The proposed methods have been applied successfully to the analysis of cited drug either in pure form or in pharmaceutical formulations with good accuracy and precision. The method herein described can be employed for quality control and routine analysis of drugs in pharmaceutical formulations.
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